STU48 has had 60 original members over 2 generations and special audition (not including Draft, Kennin and transferred members) and a total of 67 members (Plus Draft, Kennin and transferred members). As of February 2022, STU48 has 31 members and 4 Kenkyuusei.
Like in AKB48, regular auditions to recruit new members are held, and the future successful candidates, the Kenkyuusei members, take part of a in-training team of members that work as sub and under for the official members in various activities (mostly the theater performances). And then, when any of the official members graduate, a girl is promoted to fill the space.
- Bolded names indicate members, which are still active in STU48 or one of the sister groups.
- Italicized names indicate members with pending graduations.
- The members' pictures are of members who are still active in STU48 and are listed in accordance to the alphabetical order of members in each generation.
- Announced March 31, 2017
Members (31): Ishida Chiho, Ishida Minami, Isogai Kanon, Ichioka Ayumi, Imamura Mitsuki, Iwata Hina, Otani Marina, Ozaki Mami, Kai Kokoa, Kadota Momona, Kadowaki Miyuna, Kuroiwa Yui, Sakaki Miyu, Sano Haruka, Shioi Hinako, Shintani Nonoka, Sugahara Saki, Takino Yumiko, Tanaka Kouko, Taniguchi Mahina, Cho Orie, Torobu Yuri, Hyodo Aoi, Fukuda Akari, Fujiwara Azusa, Mishima Haruka, Mineyoshi Arisa, Mori Kaho, Morishita Maiha, Yano Honoka, Yabushita Fu
- Now members of STU48 1st Generation (12): Ishida Chiho, Ishida Minami, Imamura Mitsuki, Iwata Hina, Kai Kokoa, Takino Yumiko, Taniguchi Mahina, Hyodo Aoi, Fukuda Akari, Mineyoshi Arisa, Morishita Maiha, Yano Honoka
- Now Graduated (19): Kuroiwa Yui, Ozaki Mami, Cho Orie, Shioi Hinako, Sano Haruka, Sugahara Saki, Ichioka Ayumi, Kadota Momona, Torobu Yuri, Mishima Haruka, Mori Kaho, Fujiwara Azusa, Isogai Kanon, Shintani Nonoka, Tanaka Kouko, Otani Marina, Yabushita Fu, Kadowaki Miyuna, Sakaki Miyu
- Announced October 27, 2019
Members (25): Ikeda Yura, Imaizumi Miria, Utsumi Rine, Osaki Serika, Kawamata Anna, Kawamata Yuuna, Kudo Riko, Kojima Aiko, Kondo Arisu, Sako Himeka, Shimizu Sara, Suzuki Ayaka, Takao Sayaka, Taguchi Reika, Tanaka Miho, Tamura Natsuki, Nakahiro Yayoi, Harada Sayaka, Maemura Himea, Minami Yurina, Muneyuki Rika, Yoshizaki Rinko, Yoshida Sara, Rissen Momoka, Watanabe Natsuki
- Now members of STU48 2nd Generation (18): Ikeda Yura, Utsumi Rine, Osaki Serika, Kawamata Anna, Kawamata Yuuna, Kudo Riko, Kojima Aiko, Sako Himeka, Shimizu Sara, Suzuki Ayaka, Takao Sayaka, Tanaka Miho, Harada Sayaka, Muneyuki Rika, Yoshizaki Rinko, Yoshida Sara, Rissen Momoka, Watanabe Natsuki
- Now Graduated (4): Imaizumi Miria, Minami Yurina, Nakahiro Yayoi, Taguchi Reika
- Former Kenkyuusei (3): Maemura Himea, Kondo Arisu, Tamura Natsuki
- Announced in January 21, 2018
- Now members of STU48 3rd Draft (3): Oki Yuka, Shinano Soraha, Nakamura Mai
- Former Kenkyuusei (2) : Mizoguchi Aiko, Yura Akari
- Although the members are STU48's 1st Draft Generation, they are recognized as 3rd Draft for their induction in the 3rd Draft Kaigi.
New Wave Project
- Announced on May 5, 2022
- This generation is in collaboration with ASH (Actors School Hiroshima), as part of their "New Wave Project".
- Still part of Kenkyuusei (4): Okada Azumi, Okamura Rio, Kurushima Yuka, Morokuzu Noa
- Former Candidates (20): Suga Anna, Oka Yukie, Okazaki Natsumi, Ryuu Eka, Nakajima Hinano, Horibe Rina, Hirai Kana, Minami Yukari, Kikuchi Rinki, Seo Yume, Takuuru Kasako Miina, Harada Noa, Ashikaga Momoka, Haku Na, Hiyama Waka, Abe Kotomi, Ogawa Kuon, Muroi Momo, Nakamura Aika, Okuno Kokoha
- Announced February 23, 2017